| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ewing, Kennedy P (8)
|
NIGVST |
39.17 |
40.37 |
1.20 |
| 2 |
Wojciechowski, Ella G (8)
|
NIKRKN |
43.78 |
46.54 |
2.76 |
| 3 |
Leonard, Kaitllyn S (8)
|
NIWAAC |
50.17 |
47.17 |
-3.00 |
| 4 |
Miller, Nicole L (8)
|
NIHAWK |
|
47.94 |
- |
| 5 |
Blakely-Armitage, Fiona S (8)
|
NIISWM |
48.11 |
48.15 |
0.04 |
| 6 |
Jefferis, Frida R (8)
|
NIISWM |
55.04 |
55.48 |
0.44 |
| 7 |
Roberts, Olivia M (8)
|
NIHAWK |
58.40 |
56.11 |
-2.29 |
| 8 |
Knowlton, Ava L (8)
|
NIKRKN |
1:01.61 |
1:00.77 |
-0.84 |
| 9 |
Homer, Olivia K (7)
|
NIWAAC |
|
1:06.99 |
- |
| 10 |
Chin, Evelyn (6)
|
NIKRKN |
|
1:08.03 |
- |
| 11 |
Reimsnyder, Alyssa L (7)
|
NIHAWK |
|
1:14.94 |
- |
| 12 |
Walsh, Brynna M (6)
|
NIHAWK |
|
1:17.19 |
- |
| 13 |
Spencer, Danielle H (6)
|
NIWAAC |
1:50.77 |
1:22.18 |
-28.59 |
| 14 |
Fitzwater, Elizabeth O (8)
|
NIGGST |
|
1:24.55 |
- |